Medical Subject Headings (MeSH): Molecular Probe Techniques

Molecular probe techniques are laboratory methods that use specific molecules to detect and identify pathogens, genetic mutations, and other biomolecules of interest in healthcare. Some common molecular probe techniques used in healthcare include:

Polymerase Chain Reaction (PCR): A widely used technique that amplifies small amounts of DNA to make it detectable. PCR can be used to detect and identify specific pathogens, such as bacteria and viruses, and also to detect genetic mutations associated with diseases such as cancer.

Fluorescence in situ Hybridization (FISH): A technique that uses fluorescent probes to detect specific genetic sequences or chromosomal abnormalities in cells. FISH can be used to diagnose certain types of cancer, such as leukemia, and to identify chromosomal abnormalities in embryos before implantation.

DNA microarray: A technique that uses a chip or slide coated with thousands of small DNA probes to detect specific genes or genetic mutations. Microarrays can be used to identify genetic biomarkers associated with disease, and to monitor the progression of diseases like cancer.

Antigen detection: This technique uses specific antibodies to detect the presence of an antigen (a protein or carbohydrate) on the surface of a microorganism, cell or tissue. This can be used to identify specific microorganisms, for example, to identify the causative agent of an infection.

These techniques are widely used in diagnostic laboratories, clinical research and can also be used for monitoring treatment response and disease progression.

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